subtilis B-916 strain, and after obtaining an active elution peak, the protein spectrum identified a novel antibacterial protein, Bacisubin, with a molecular weight of 41.900 kDa. ( 2007) used a DEAE-Sepharose Fast Flow weak ion exchange column to chromatograph the crude protein secreted by B. Rahnella aquatilis JZ-GX1 produces volatile gases that antagonize Colletotrichum gloeosporioides, which causes Liriodendron chinense × tulipifera black spot, by containing 3-methyl-1-butanol and 2-methyl phenyl ether ethyl ether (Kong et al. Fengycin from Bacillus velezensis achieved better inhibition of mycelial growth in Fusarium solani than chemical fungicides, and its activity remained stable even at high temperatures and low pH values (Wang et al. campestris and by causing swelling and roughness of the cell surface of Xanthomonas axonopodis pv. The mixture of iturin and surfactant from Bacillus subtilis OG strain exerted antagonistic effects by inducing destruction of the cell wall, shrinkage, and leakage of intracellular content in Xanthomonas campestris pv. Researchers have identified a variety of antagonistic substances from different biocontrol strains, mainly including two categories: antagonistic substances synthesized by the ribosomal synthesis pathway, including bacteriocins, cell wall degrading enzymes and antimicrobial proteins and lipopeptide antibiotics synthesized by the non-ribosomal synthesis pathway, including bacilysin, surfactin, iturin, fengycin and kurstakin, etc. Antagonism refers to the growth process of antifungal substances produced by the bacterium to inhibit the mycelial growth and spore germination of the pathogen, disrupting the normal physiological metabolism of the mycelial cells and the synthesis of cellular inclusions, and eventually leading to the normal growth or even death of the pathogen (Alina et al. Bacillus can control plant diseases through a variety of mechanisms, the most direct way is antagonism. has received much attention from researchers because of its high resistance, wide range of habitat and high safety, and plays an important role in the biological control of plant diseases (Berg 2009 Ongena and Jacques 2008). pumilus HR10.Īt present, among many biological control microorganisms, Bacillus spp. pumilus HR10 were further isolated and identified using HiTrap Capto Q strong Ion-Exchange Chromatography and LC–MS-MS, and it was speculated that glycoside hydrolase (Ghy), beta-glucanase (Beta), arabinogalactan endonuclease β-1,4-galactanase (Arab), and immunosuppressant A (ImA) are proteins with antagonistic activity against S. pumilus HR10 cultured with the optimized medium formulation was verified to have an inhibition rate of (87.04 ± 3.2) % on the growth of Sphaeropsis sapinea by three replicate tests. pumilus HR10 was 12 g/L corn meal, 15 g/L beef extract and 13 g/L magnesium sulfate, with a predicted bacterial inhibition rate of 89%. pumilus HR10 was finally obtained by single-factor test, Packett-Burman test, steepest ascent test and Box-Behnken Design (BBD) response surface test, and the best formulation of the fermentation medium for the antagonistic substance of B. The optimal formulation of the fermentation medium for the antagonistic substance of B. A previous study found that a biocontrol bacterium, Bacillus pumilus HR10, inhibited the Sphaeropsis shoot blight disease of pine, and the fermentation broth of HR10 strain contained protein antifungal substances.
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